Information on proteins involved in miRNA processing


Protein Motifs Function Organism OR Cell System Pubmed
Dicer RNase III, DEAD, PAZ, dsRBD   miRNA precursor processing   H. sapiens   15372041   
DCR-1 RNase III, DEAD, PAZ, dsRBD   miRNA precursor processing   C. elegans   15372041   
DCR-1 RNase III, HELICc, PAZ, dsRBD   miRNA precursor processing   D. melanogaster   15372041   
DCL1 RNase III, DEAD, PAZ, dsRBD   miRNA precursor processing   A. thaliana   15372041   
AGO1 PAZ, PIWI   Short RNA binding   H. sapiens, D. melanogaster   15372041   
AGO2 PAZ, PIWI   Short RNA binding   H. sapiens, D. melanogaster   15372041   
AGO3 PAZ, PIWI   Short RNA binding   H. sapiens   15372041   
AGO4 PAZ, PIWI   Short RNA binding   H. sapiens   15372041   
AGO1 PAZ, PIWI      A. thaliana   15372041   
ALG-1/2 PAZ, PIWI      C. elegans   15372041   
Gemin3 DEAD   RNA helicase   H. sapiens   15372041   
Armitage DEAD   RISC assembly   D. melanogaster   15372041   
Drosha RNase III   Processing of primary miRNA transcript   H. sapiens   15372041   
Exportin-5 NA   Nuclear export of miRNA precusors   H. sapiens   15372041   
Gemin4 NA      H. sapiens   15372041   
FMRP, FXR KH, RGG   RNA binding   H. sapiens, D. melanogaster   15372041   
Ribosomal Proteins L8, L11 NA   60S ribosomal subunit   D. melanogaster   15372041   
HYL1 dsRBD      A. thaliana   15372041   
HEN1 NA      A. thaliana   15372041   
ADAR1, ADAR2 dsRNA   Editing of pri-miRNA miR-22
miR-151, -197, -223, -376a, -379, 99a
miR-142, -223, -1-1, -143
  
M. musculus
H. Sapiens
M. Musculus
  
15272117
16594986
16369484
  
P72, P68 DEAD-box   Might act as specificity factors for the processing of a subset of pri-miRNAs   M. musculus   17435748  
TGF-beta, BMPs, SMADs R-Smad, Co-Smad   Induces SMAD signaling to the miR-21 precursor and enhances its efficient processing by Drosha   H. sapiens   18548003
19892582
  
Lariat debranching enzyme    Processing of mirtrons   D.melanogaster, C.elegans   17964270
17599402
17589500
  
Lin-28    Facilitates the expression of mature let-7 during embryonic stem cell differentiation   Embryonic/Stem cells   18951094
18292307
18604195
18566191
18550544
  
TRBP    Stabilizes Dicer   H. sapiens   16142218
16424907
18178619
  
Hexa nucleotide terminal motif hnt motif   Imports hsa-miR-29b into nucleus   H. sapiens   17204650  
NRDE-3    Nuclear import   C. elegans   18653886  
Importin-8    Required for cytoplasmic miRNA-guided gene silencing and affects nuclear localization of Ago proteins   H. sapiens   19167051  
FIERY1, XRN2, XRN3    Degrades loop sequence released from pre-miRNA after Dicer cleavage   A. thaliana   17993620  
XRN2    Degrades mature miRNAs   C. elegans   19734881  
TUT4/Zcchc11/PUP-2    Destabilization of miR-122 and Reduction of function, but not stability of miR-26b   C. elegans,M. musculus   19713957
19713958
19703396
  
SDN1    Degrades mature miRNAs   A. thaliana   18787168  
c-myc helix-loop-helix   Activates transcription of miR-17-92 clustery
Decreases the expression of miR-15a, -29, -34, and let-7 families (tumor supressor miRNA genes)
  
Lymphoma cell   15944709
18066065
  
P53 DNA binding domain   Activates transcription of miR-34 family   M. musculus   17540598
17554337
  
KSRP G-rich region   Promotes both Drosha and Dicer mediated miRNA processing and required for the maturation of let-7a and miR-206   Hela cells   19458619  
NF90/NF45 small dsRNAs   Repression of processing of let-7 family, and reduce pre-miRNA and increases pri-miRNA levels   Hela cells   19398578
14570900
  
TRIM-NHL family    TRIM32 enhance the activation of miRNA(let-7a)
NHL-2 enhance the activation of miRNAs
  
M. musculus
C.elegans
  
19269368
19269369
  
ELAV1 AU-rich element   inhibit miR-122 repression of target sites   H. sapiens   16777601  
Dnd1 U-rich region   inhibit miRNA access to target mRNA   H. sapiens, Zebrafish   18155131  

Information on proteins linked with human or mouse Agos


Argonaute Protein Domains/Motifs Function in Gene Sliencing Cellular Localization Pubmed
hsAgo1  
Dicer   DExH box, PAZ, RNaseIII, dsRBD   Small RNA Processing   Cytoplasm   16289642  
TNRC6B   RRM, GW repeats   miRNA-guided cleavage   Cytoplasm, P bodies   16289642  
DCP1a      Not investigated   Cytoplasm, P bodies   15937477  
DCP2   Nudix   Not investigated   Cytoplasm, P bodies   15937477  
MOV10   DExH, box   miRNA-guided cleavage   Cytoplasm, P bodies   16289642  
PRMT5   Methyl-transferase   Not investigated   Cytoplasm, Nucleus   16289642  
hsAgo2  
Dicer   DExH box, PAZ, RNaseIII, dsRBD   Small RNA Processing   Cytoplasm   16289642
16271387  
TNRC6A/GW182   RRM, GW repeats   miRNA-guided cleavage, translational repression   Cytoplasm, P bodies   16284622
16284623  
TNRC6B   RRM, GW repeats   miRNA-guided cleavage   Cytoplasm, P bodies   16289642  
DCP1a      Not investigated   Cytoplasm, P bodies   15937477  
DCP2   Nudix   Not investigated   Cytoplasm, P bodies   15937477  
TTP   Zn-finger   AU-rich mRNA destablization   Cytoplasm   15766526  
MOV10   DExH box   miRNA-guided cleavage   Cytoplasm, P bodies   16289642  
PRMT5   Methyl-transferase   Not investigated   Cytoplasm, P bodies   16289642  
eIF4E      Not investigated   Nucleus, Cytoplasm, P bodies   16756390  
Rck/p54   DEAD box   miRNA-guided cleavage   Cytoplasm, P bodies   16756390  
Gemin3   DEAD box   miRNA-guided cleavage   Nucleus, Cytoplasm   11914277  
Gemin4      Not investigated   Nucleus, Cytoplasm   11914277  
PACT   dsRBD   Small RNA Processing, RISC activity   Cytoplasm   16424907  
FMRp   KH domain, RGG box   Not investigated   Nucleus, Cytoplasm   14703574  
FXR1   KH domain, RGG box   Not investigated   Nucleus, Cytoplasm   14703574  
FXR2   KH domain, RGG box   Not investigated   Nucleus, Cytoplasm   14703574  
hsAgo3  
Dicer   DExH box, PAZ, RNaseIII, dsRBD   Small RNA Processing   Cytoplasm   16289642  
hsAgo4  
Dicer   DExH box, PAZ, RNaseIII, dsRBD   Small RNA Processing   Cytoplasm   16289642  
TTP   Zn-finger   AU-rich mRNA destablization   Cytoplasm   15766526  
MIWI  
Dicer   DExH box, PAZ, RNaseIII, dsRBD   Small RNA Processing   Cytoplasm   16477042  
KIF17b   Kinesin motor   Not investigated   Cytoplasm, Chromatoid body   16787948  
MVH   DEAD box   Not investigated   Cytoplasm, Chromatoid body   14736746  
MAEL   HMG box   Not investigated   Cytoplasm, Chromatoid body, XY-body, Nucleus   16787967  
MILI  
MAEL   HMG box   Not investigated   Cytoplasm, Chromatoid body, XY-body, Nucleus   16787967